Higher Incidence of Chromosomes 13, 18, 21, X and Y Aneuploidy in Spermatozoa from Patients with Severe Oligospermia and Repeated ICSI Failure Versus Fertile Control
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- Category: Vol. 77, June 2009
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Higher Incidence of Chromosomes 13, 18, 21, X and Y Aneuploidy in Spermatozoa from Patients with Severe Oligospermia and Repeated ICSI Failure Versus Fertile Control,SHERIF A. GHAZI, YASSER A. KHYIAT, AMR M. GADALLA, AHMAD M. SEIF, SHEDID A. SHEDID and HOWIDA K. ABDULFATTAH
Abstract
The incidence of numerical chromosomal abnormalities is higher in spermatozoa compared with somatic cells in normal population. The aim of this study is to examine the incidence of sperm aneuploidy for the chromosomes 13, 18, 21, X and Y using dual colour FISH technique in two groups of men. Group A: are 14 infertile men with severe oligospermia (sperm density less than 2 million sperms/ml) and had history of repeated ICSI failure. Group B: 8 fertile men with normal semen parameters. The mean total aneuploidy rate in men of group (A) is significantly higher than the fertile men of group (B) (3.18 Vs 0.73) (p<0.01). The mean aneuploidy rate for each examined chromosome was significantly higher in group (A) compared with group (B). The highest aneuploidy rate affected the gonosomes (X & Y). This results support the hypothesis that increased incidence of chromosomal aneuploidy has a negative effect on embryonic development and implan-tation rate. Screening of men attempting ICSI procedure for sperm chromosomes aneuploidy is a potential prognostic tool.