Identification of Fas-Targeting siRNA Expression in Aplastic Anaemia
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- Category: Vol. 77, June 2009
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Identification of Fas-Targeting siRNA Expression in Aplastic Anaemia,OLA M. EL-DESSOUKY, HANAA G.M. SAYED and NIVEEN M. SALAMA
Abstract
Introduction: Aplastic anemia (AA) is a bone marrow failure syndrome characterized by an immune mediated de-struction of marrow haematopoietic progenitor/stem cells. It was almost universally fatal just a few decades ago, but now aplastic anemia can be cured or ameleorated by many thera-peutic trials (e.g. stem cell transplantation and immuno suppressive drug therapy).
The aim of this study: Is to examine the contribution of fas and fas inhibition by siRNA (small interfering RNA) pathogenesis in cases of fanconi and non fanconi aplastic anaemia.
Patients and Methods: This study included 20 patients, 9/20 were fanconi aplastic anemia (45%) and 11/20 (55%) were non fanconi aplastic anemia their ages ranged from 6 years to 23 years with a mean of 14.17±5.2, they were 7 females and 13 males, this study included also 10 healthy volunteers with age and sex matched controls. Molecular analysis of the siRNA against FAS gene for patients and control groups were investigated using RT PCR (Reverse Transcriptase Polymerase Chain Reaction).
Results: All studied cases whether fanconi or non fanconi aplastic anemia revealed positive result. In contrast non aplastic anemia control samples showed -ve results at the specified band.
Conclusion: Expression of FAS antigen in chronic aplastic anemia whether fanconi or non fanconi using RT-PCR reverse transcriptase polymerase chain reaction and is counteracted by self regulatory siRNA. This suggests the possible beneficial role of RNA interference in regulated doses for treatment of chronic aplastic anemia.