Protective Effect of Xanthine Oxidase Inhibition on Ischemia/Reperfusion Injury in Rat Liver
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- Category: Vol. 79, December 2011
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Protective Effect of Xanthine Oxidase Inhibition on Ischemia/Reperfusion Injury in Rat Liver,EFFAT A. KHOWAILED, HANAN A. MUBARAK, HANAN A. SEDDEEK and NANCY F. SAMIR
Abstract
Ischaemia reperfusion (I/R) injury is a major cause of morbidity and mortality in patients undergoing partial liver resection or transplantation. Despite the ongoing research identifying novel approaches of protection against I/R injury such as pharmacologic, genetic & surgical approaches, there is still no accepted therapy used in the clinical transplantation to minimize liver injury. In this study we examined the effects of allopurinol (Allo), ischemic preconditioning (IPC) and postconditioning (IPO) on the extent of I/R injury of rat liver in a trial to evaluate their role as a protective strategy against hepatic I/R injury. The study was conducted on 70 adult male albino rats that were divided into the following 5 main groups: Group I: Control sham-operated group, Group II (I/R): Hepatic ischaemia reperfusion group, Group III: Allopurinol–treated (Allo+I/R) group, Group IV: Conditioning group which in-cluded ischemic preconditioning (IPC+IR) subgroup and ischemic postconditioning (IPO+IR) subgroup, Group V: combined allopurinol and conditioning group which included (Allo+IPC+IR) subgroup and (ALLO+IR+IPO) subgroup. Serum alanine aminotransferase (ALT) enzyme, liver tissue malondialdehyde (MDA) and reduced form of glutathione (GSH) were measured. Results revealed significant decrease of serum ALT and liver MDA with significant increase in liver GSH in (Allo+I/R), (IPC+IR), (IPO+IR), (Allo+IPC+IR) and (ALLO+IR+IPO) groups compared to IR group. In con-clusion, there are protective and additive effects of allopurinol & different conditioning protocols on I/R injury in rat liver.