Experimental Staining of the Anterior Lens Capsule in Albino Rabbits Using Capsulorhexis Marker
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- Category: Vol. 79, March 2011
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Experimental Staining of the Anterior Lens Capsule in Albino Rabbits Using Capsulorhexis Marker,MOHAMED A. HASSABALLA
Abstract
Purpose: The aim of this study was to assess the toxicity of gentian violet, applied directly on the anterior capsule by the lower edge of capsulorhexis marker, to intraocular struc-tures especially the corneal endothelium and retina.
Setting: Wet Lab. Kasr El-Aini Hospital.
Methods: This prospective comparative experimental study included 20 eyes of 10 albino rabbits that were assigned into two groups; group 1 (experimental, in which the gentian violet was used) and group 2 (control, without gentian violet). The right eye of each rabbit was put in group 1 and the left eye was put in group 2. The animals were divided into 2 subgroups; the first was killed 1 day after surgery and the second, after 1 week. The eye balls were enucleated and immediately fixed in bouin’s solution and embedded in par-affin. It was examined by light microscope.
Results: Light microscopy examination of multiple sections from each eye showed normal cornea iris tissue, ciliary body epithelium and lens epithelium. In one eye of the study group, there was loss of ganglion cells from neurosensory retina one day after surgery.
Conclusions: Staining the anterior capsule by gentian violet using the capsulorhexis marker is a relatively safe procedure with minimal histologic abnormalities. Further studies are needed using larger number of animals to exclude retinal toxicity.