Influence of Helicobacter Pylori Infection on Plasma Ghrelin Level in Patients with Upper GIT Lesions
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- Category: Vol. 79, March 2011
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Influence of Helicobacter Pylori Infection on Plasma Ghrelin Level in Patients with Upper GIT Lesions,AHMED A. HIEKAL, WAEL SHAWKY, MOHAMED S. ABDALLA, DALIA I. RAMADAN and HAYTHAM A. EL-NAGGAR
Abstract
Background: The stomach is the main source of circulating ghrelin. Gastric colonization by H. pylori showed to affect ghrelin dynamics. This fact is due to long-term persistent H. pylori infections leading to atrophic gastritis. Some authors reported that; the relation between H. pylori status and ghrelin level is uncertain.
Aim of Work: This study evaluates the effect of H. pylori infection on gastric ghrelin production and consequently the plasma ghrelin concentration.
Subjects and Methods: Forty patients complaining of abdominal pain, dyspepsia, acid regurge and burning pain were included in the study after giving informed consent. Estimation of ghrelin hormone level and H. pylori IgG antibody was done. The detection of ghrelin level was based on com-petitive enzyme immunoassay. While the detection of H. pylori was performed using quantitative estimation of H. pylori IgG, in addition to endoscopic biopsies from antrum and corpus of the stomach. All specimens were subjected to histopathological examination using hematoxylin and eosin stain to detect H. pylori.
Results: Ghrelin level is higher among subjects with H. pylori positive than H. pylori negative regarding the mean and individual data persons. With median in H. pylori negative (n=8) is 173.5 (44.95-589.25). Median in H. pylori positive (n=32) is 614 (253-928.38) p-value=0.052, borderline signif-icant. As regards H. pylori; it was detected pathologically with hematoxylin and Eosin and serologically by IgG antibody detection. The pathological specimen showed that; out of 40 patients 8 (20%) were negative, while 32 patients (80%) were positive for H. pylori. On the other hand by H. pylori IgG detection on the same patients 8 patients were below 15 (non-reactive) i.e. –ve, meanwhile the remaining 32 were above 15 (reactive) i.e. +ve. So there is significant association between the two methods for detection of H. pylori p<0.001.
Conclusion: Marked discrepancies do exist in the result about ghrelin plasma level and H. pylori colonization has been associated with high, equivalent or low level of plasma ghrelin. So the relation between H. pylori status and ghrelin levels is uncertain. Regarding the diagnosis of H. pylori infection, it was concluded that sensitivity of H. pylori IgG antibodies versus histopathological examination was 100% and specificity was 100%, since the result was the same in diagnosis.