Contribution to A New Potential Role of Oxytocin in Combating Atherogenic Events in Hyperlipidemic Castrated Male Rats
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- Category: Vol. 80, September 2012
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Contribution to A New Potential Role of Oxytocin in Combating Atherogenic Events in Hyperlipidemic Castrated Male Rats,MAGDA M. EL-HAMZAWY, HEBA M. SHAWKY, HANY EL-SEBAIE, HEBA S. SHOUKRY, LAILA A. RASHED, MAHA B. ZICKRI and HALA GABR
Abstract
Oxytocin was determined in many previous studies to be involved in mechanisms related to attenuation of cardiovascular disorders. We aimed in this study to investigate the role of oxytocin in weakening the atherosclerotic events. To achieve this, the present experiment involuted the main group of hyperlipidemic male rats subjected to high-fat diet regimen for 8 weeks. The second group was subjected to surgical bilateral orchiectomy. Blood samples were withdrown to measure the serum lipid profiles, high sensitive C-reactive protein (CRP), IL-6 and adiponectin levels. Biochemical analysis of gene expressed levels of IL-6, ER-alpha and adiponectin receptors 1&2 (ADIPR1, ADIPR2) in the adipose as well as vascular tissue were assessed, in addition to histo-pathological and morphometeric estimation of associated signs of inflammatory cell infiltration in these tissues. After 8 weeks, Oxytocin therapy in our thesis displayed a potent role in the improvement of atherosclerosis through the highly significant reduction in the level of atherosclerotic markers, body weight and visceral fat in oxytocin-treated group in comparison to castrated-saline treated group. Moreover, there was a significant reduction in the inflammatory markers and Il-6 gene expression. Our results displayed a significant increase in the level of serum adiponectin, ER-a and adiponec-tin receptors 1&2 gene expressed levels in oxytocin-treated group in comparison to castrated-saline treated and sham-operted groups, a significant improvement in the histopatho-logical results of the aortic and adipose tissue after adminis-tration of oxytocin, through a significant reduction in the level of infiltrating inflammatory cells and reduction in the level of apoptotic nuclei. As regards the adipose tissue, there was also a significant reduction in the infiltrating cells as well as significant reduction in the size of adipocytes and the vessels congestion.
In Conclusion: We provide evidence that oxytocin could prevent the stress that induce the atherosclerotic events thus attenuating its pathogenesis as an anti-stressor. Moreover, peripheral oxytocin administration was shown to act as an anti-inflammatory, anti-oxidant and anti-fibrotic agent.