A Genomic Study of Type 2 Diabetics Attending the Outpatient Clinic of National Institute of Diabetes and Endocrinology
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- Category: Vol. 81, March 2013
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A Genomic Study of Type 2 Diabetics Attending the Outpatient Clinic of National Institute of Diabetes and Endocrinology,ASHRAF ISMAIL, GHADA HUSSEIN, GHADA A. OMAR, AYAT I.GHANEM and MOHSEN KHALID
Abstract
Background: A panel of established variant single nucle-otide polymorphisms (SNPs) in patient diagnosed with type 2 diabetes mellitus (T2DM) is being evaluated within many studies compared to a non diabetic control population. These results will be used as a basis of comparison to analyze risk -conferring genotypes in T2DM to demonstrate T2DM risk associated factors.
Aim of the work: This study was designed to study the most common SNPs associated with type 2 diabetes to identify genetic markers in type 2 diabetic patients attending in the outpatient clinic of National Institute of Diabetes & Endo-crinology (NIDE).
Subjects and Methods: This study was conducted on a total number of 71 subjects which were subdivided as follows: Group I: Included 49 type 2 diabetic subjects were selected from the outpatient clinic of National Institute of Diabetes and Endocrinology (NIDE), Group II: Included 22 normal healthy subjects (as controls), matching the same age and sex. In addition to the routine investigation the following SNPs genotypes were studied:
(rs10010131, rs7754840, rs4402960/rs1470579, rs13266634 and rs10923931).
Results: The IGF2BP2 rs4402960/rs1470579 polymor-phism showed the highest odds ratio (OR) for type 2 diabetes group (4.714). Odds ratio of other polymorphisms ranged from 1.131 to 4.270. The logistic regression used to assess the contribution of individual SNPs to risk of type 2 diabetes showed that the IGF2BP2 rs4402960/rs 1470579 polymor-phism showed significant risk of type 2 diabetes, p-value is less than 0.05 and this mean that we have evidence against HO (HO: coefficients=0). The other polymorphisms (WFS1 rs10010131, CDKAL1 rs7754840, SLC30A8 rs13266634, NOTCH2 rs10923931) present with p-value which is greater than 0.05 and this mean that we have little evidence against HO, i.e. we accept HO, thus (WFS1 rs10010131, CDKAL1 rs7754840, SLC30A8 rs13266634, NOTCH2 rs10923931) are showed insignificant risk of type 2 diabetes. Within positive cases to SNPs IGF2BP2 rs4402960/rs1470579, there are significant difference in triglycerides between two allele positive cases and one allele positive cases.
Conclusion: It can be concluded that SNPs (IGF2BP2 rs4402960/rs1470579) one of the evaluated 5 SNPs was found to be associated with an enhanced risk of future diabetes and prediction of future disease in diabetic Egyptian patients. This finding can be used in the prediction, prevention and early detection of the disease especially if the study applied to large scale.