Effect of Exercise on 11ß-Hydroxysteroid Dehydrogenase Type 1 Expression in Adipose Tissue of Ovariectomized Rats
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- Category: Vol. 81, September 2013
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Effect of Exercise on 11ß-Hydroxysteroid Dehydrogenase Type 1 Expression in Adipose Tissue of Ovariectomized Rats, HANY EL SEBAEE, ZAINAB ABDEL WAHAB, SANDRA YOUNAN, LAILA A. RASHED and NANCY F. SAMIR
Abstract
Objective: With ovariectomy or menopause in women there is a shift in adipose tissue distribution from subcutaneous to visceral depots. Thus, in the present work we studied the possible role of 11/3-HSD1 enzyme in redistribution of adipose tissue in ovariectomized female rats. Moreover, we tried to clarify if exercise training has similar regulatory roles on 1113- HSD1 expression in adipose tissue of ovariectomized rats as estrogen replacement.
Material and Methods: Female albino rats (180-200g) were randomly divided into Group 1: Control-sham operated group; Group 11: Ovariectomized (OVX); Group III: Ovariec-tomized + estradiol therapy (OVX+E2); Group IV: Ovariec-tomized + exercise (OVX+Ex). Six weeks after ovariectomy animals were weighed and blood samples were collected. Then, animals were sacrificed and subcutaneous and visceral adipose tissue depots were dissected. Visceral fat depot weight was measured. Plasma estradiol and corticosterone levels were measured while Corticosterone level; 11/3-HSD1 gene expression and Glucocorticoid receptor gene expression were measured in subcutaneous and visceral adipose tissue.
Results: OVX group showed significant rise in body weight and visceral fat depot weight compared to control group. While, estradiol injection and exercise significantly decreased body weight and visceral fat depot weight compared to OVX group. Corticosterone levels in plasma, SCAT and VAT were significanty elevated in OVX group compared to control group. Values were significantly reduced by estradiol injection and exercise. The 11/3-HSD1 and GR gene expressions in SCAT and VAT were significantly elevated in OVX group compared to control group. Estradiol injection and exercise could significantly lower GR gene expression level in SCAT. However, 11/3-HSD1 gene expression level was significantly lowered by estradiol but not by exercise. On the other hand, estradiol injection and exercise could significantly lower 11/3- HSD1 & GR gene expression in VAT compared to OVX group.
Conclusion: Ovariectomy results in an increase in body weight and visceral adiposity partly caused by an elevation of 1113-HSD1 gene expression in subcutaneous and visceral adipose tissue. Estradiol replacement or exercise, both have a protective effect against the observed increase in body weight and visceral adiposity and could significantly attenuate the rise in the 11/3-HSD1 gene expression in visceral adipose tissue.