Influence of the Phosphodiesterase Type 5 Inhibitor, Sildenafil, on Some Behavioral and Central Biochemical Changes on Chronic Restraint Stress in Rats
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- Category: Vol. 82, September 2014
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Influence of the Phosphodiesterase Type 5 Inhibitor, Sildenafil, on Some Behavioral and Central Biochemical Changes on Chronic Restraint Stress in Rats, SUZAN M. HAZZAA, SUZY F.EWIDA and MAHA ELBATSH
Abstract
Stress and anxiety are now considered as major health problems that may impair the brain ability to regulate physi-ological and behavioral responses to stress. Disturbed serotonin and oxidative stress have been linked to anxiety and depression. This study aimed to demonstrate the effect of sildenafil (phosphodiestrase 5 inhibitor) on anxiety like behavior in chronic restrained rats and to elucidate the rule of hippocampal serotonin and oxidative stress. Fifty adult male albino rats of the same age were divided into five equal groups: Control group, chronic restraint-stressed group (CRS): Stress was induced by repeated restraint of rats in plastic tubes 2h/21 days, Sildenafil-treated group: CRS rats were treated by sildenafil, Atropine-treated group: In which CRS rats were treated by atropine, Combined treated group: CRS rats were treated by both sildenafil and atropine. One day after the final restraint stress, locomotor activity and behavior were tested using open field and elevated plus maze tests. After behavioral assessment, rats were sacrificed by cervical decapitation. Fresh brains were taken hippocampus was identified and dissected then used for preparation of homogenate. These homogenates were used for estimation of serotonin level, malondialdehyde (MDA) and super oxide dismutase (SOD). CRS was found to decrease brain serotonin level, produce oxidative stress as indicated by increased level of MDA and decreased level of SOD. Treatment with sildenafil alone or combined treatment with sildenafil and atropine were found to increase the brain serotonin levels and SOD with decrease in MDA levels. Treatment with atropine alone did not cause significant changes in the measured parameters. It was found that CRS induced a state of anxiety with oxidative stress response. Sildenafil and combined treatment had an anxiolytic and antioxidant effects on CRS.