Immunohistochemical Expression of Inducible Nitric Oxide Synthase and Distribution of Secretory Cells in Human Fallopian Tube
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- Category: Vol. 82, December 2014
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Immunohistochemical Expression of Inducible Nitric Oxide Synthase and Distribution of Secretory Cells in Human Fallopian Tube, RASHA I. ANWAR and MOHAMED A. ELADL
Abstract
Background: The human fallopian tube provides an envi-ronment that enhances and supports fertilization and early embryonic development. Inducible nitric oxide synthase (iNOS) has been suggested that it participates in the regulation of tubal function. Additionally, secretions of secretory cells contain nutrients for spermatozoa, oocytes, and zygotes. The secretions also promote capacitation of the sperm.
Aim of the Work: To examine expression of iNOS and distribution of the secretory cells in the ampulla and ithmus of human fallopian tube during various stages of the repro-ductive and the postmenopausal periods.
Material and Methods: Fallopian tubes were obtained from 36 women at proliferative, secretory and postmenopausal periods. Samples were collected from the isthmus and the ampulla. Slides were stained with Haematoxyline & Eosin and immunohistochemical demonstration of iNOS. The fol-lowing parameters were done; counting of the secretory cells and the area percent of expression of iNOS in the isthmus and the ampulla.
Results: During the proliferative phase, the mucosa was relatively thick, and covered by crowded columnar epithelial cells; the cells were of 2 types ciliated and non ciliated (secretory). Ciliated cells were more obvious in the ampulla than the isthmus. While secretory cells appeared more frequent in the isthmus as compared to the ampulla especially during the secretory phase. This was confirmed by counting of the secretory cells which showed a significant increase in the number of the secretory cells in the isthmic epithelium during the secretory phase as compared to the ampullary epithelium. iNOS expression was more intense in the isthmus specially during the secretory and the postmenopausal phases. Statisti-cally, the area % of iNOS expression showed significant difference in the different phases.
Conclusion: The results of this study revealed difference in iNOS expression between the isthmus and the ampulla at the different phases which suggest a different role of NO in the ampulla and the isthmus regions. At the same time, the distribution of the secretory cells at the isthmus was more as compared to the ampulla which is important for fertilized ovum nutrition. Further studies needed to investigate the role of iNOS in the postmenopausal phase.