Possible Protective Effect of Thyroid Hormone on Hepatic Ischemia Reperfusion Injury in Rats
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- Category: Vol. 84, June 2016
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Possible Protective Effect of Thyroid Hormone on Hepatic Ischemia Reperfusion Injury in Rats, MOHAMMED A. ABD AL-HAFEZ, MIRA F. YOUSSEF, DALIA I. ISMAIL, NERMEEN BASTAWY and HANAN A. HASSAN
Abstract
Background: Ischemia Reperfusion Injury (IRI) is one of the major complications affecting the outcomes of liver surgical interventions. This study aimed at evaluating the possible protective effects of Thyroid Hormone (TH) on IRI in the liver of adult male albino rats.
Methods: Forty male rats were included and divided equally into 4 groups: Group I (sham operated control group); Group II: Received TH 48 hours before sham operation; Group III: Induction of hepatic IRI as 45min. of ischemia and 4 hours of reperfusion; Group IV: Received TH 48 hours before IRI. Before sacrification, serum AST and ALT levels were measured. Liver sections were stained with H & E, and immunohistochemical stain for anti-CD68.
Results: In TH group, there was no significant difference in AST and ALT as compared to the control. Also the histo-logical architecture of the liver appeared as in control rats. The mean area % of anti-CD68 immunoreactivity showed no significant difference as compared to the control. IRI group showed significant increase in AST and ALT when compared to other groups. Liver sections revealed disorganization, degeneration and necrosis of the hepatocytes with significant increase in the mean area % of anti-CD68 immunoreactivity compared to control. In TH & IRI group, there was significant decrease in AST & ALT when compared to IRI group. Liver sections showed decrease in the histological alterations caused by IR. There was significant decrease in the mean area anti-CD68 immunoreactivity when compared to Group III.
Conclusion: TH has a protective effect against hepatic IRI.