Potential Effect of Mesenchymal Stem Cells and/or Vitamin D on Hepatoma Cell Line
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- Category: Vol. 84, December 2016
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Potential Effect of Mesenchymal Stem Cells and/or Vitamin D on Hepatoma Cell Line, HANAN FOUAD, LAILA RASHED, RANIA E. HUSSEIN and SHIMAA SAAD EL-DIN
Abstract
Objective: This study was conducted to evaluate the effect of mesenchymal stem cells (MSCs) and/or vitamin D on apoptosis and cell proliferation of HepG2 cells (hepatoma cell line).
Methods: In this study, HepG2 cells were divided into: HepG2 control group, HepG2 cells treated with MSCs-conditioned medium (MSCs-CM) only, HepG2 cells treated with vitamin D only, and HepG2 cells treated with both MSCs-CM and vitamin D. Quantitative measurement of gene expres-sion of Bax and Bcl-2 by RT-PCR, in addition to HepG2 cell proliferation assay were performed in all groups.
Results: All treated HepG2 cells showed a significant increase in Bax and significant decrease in Bcl-2 gene expres-sion, in addition to significant decrease in HepG2 cell prolif-eration rate.
Conclusion: Adminstration of MSCs, vitamin D or both promotes apoptosis, in vitro, in HepG2 cell lines through upregulation of Bax gene and downregulation of Bcl-2 gene expression.