Effect of Diclofenac on the Intraocular Pressure Lowering Action of Latanoprost in AlbinoRabbits with Induced Ocular Hypertension
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- Category: Vol. 84, December 2016
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Effect of Diclofenac on the Intraocular Pressure Lowering Action of Latanoprost in AlbinoRabbits with Induced Ocular Hypertension, GHADA G. EL-HOSSARY, AMAL A. EL-GOHARY, LAILA H.M. EL-SHAZLY and AMANY H.M. EL-SHAZLY
Abstract
Aim: The present work aims to test the effect of the COX inhibitor diclofenac on the intraocular pressure (IOP)-lowering action of the prostaglandin analog, latanoprost.
Methods: Twelve albino rabbits were divided into four groups with six eyes in each group. Group I served as control. Ocular hypertension was induced in groups II, III and IV by a single subconjunctival injection of betamethasone (0.5ml, 7mg/ml) on day 1 and animals were followed-up for 6 days. Group II was left untreated. In group IV, diclofenac was applied topically 4 times on 5th and 6th postinduction days. On day 6, in groups III and IV, latanoprost was applied topically once to rabbit eyes and the IOP was measured before application and 15min, 30min, 1h, 2h, 3h and 4h after latan-oprost application. Photopic electroretinogram (ERG) record-ings were performed for all groups before the experiment and on day 6 post betamethasone injection measuring b-wave amplitude, full-field photopic negative response (PhNR) amplitude and PhNR/b-wave ratio.
Results: The IOP was significantly elevated in groups II, III and IV reaching 28.8±3.38, 26.28±3.34 and 29.93± 2.04 mmHg, respectively compared to control (16.55±2.86mmHg) on the 6th day. In group III, latanoprost application produced significant reduction of IOP reaching 15.07±2.96mmHg four hours after latanoprost with improvement of ERG parameters. In group IV, pretreatment with diclofenac antagonized the IOP-lowering effect of latanoprost and prevented the improve-ment of ERG parameters.
Conclusion: Diclofenac can antagonize the IOP-lowering effect of latanoprost. Therefore, careful follow-up of IOP is required when these two drugs are co-administered.