Obesity Related Colorectal Cancer through Insulin Like Growth Factor Binding Protein Axis and Gene Polymorphism in Egyptians
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- Category: Vol. 84, December 2016
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Obesity Related Colorectal Cancer through Insulin Like Growth Factor Binding Protein Axis and Gene Polymorphism in Egyptians, AYMAN YOSRY, MOHAMAD YOUSEF, MOHAMAD SALAH, MOHAMAD S. MARIE, HEBA OMAR, SHERIF HAMDY, OLFAT SHAKER and DALIA OMRAN
Abstract
Background and Aim: Insulin pathway plays a role in mediation of Colorectal Cancer (CRC). In this study, we investigated the association between CRC and obesity in Egyptians through assessment of serum levels of IGFBP-3 in Egyptian CRC patients. We also studied Single Nucleotide Polymorphism (SNP) in Insulin-like Growth Factor Binding Protein (IGFBP) gene in Egyptian CRC patients with different BMI.
Methods: This prospective study included 66 CRC patients and 30 healthy individuals, for whom Body Mass Index (BMI) was estimated, patients and controls were categorized into overweight or obese as one group and average weight as another group. Serum levels of IGFBP-3 were assessed by ELISA and SNP in IGFBP gene at rs2854744A/C & rs2854746 C/G were studied by PCR-RFLP.
Results: Serum level of IGFBP3 was significantly higher in CRC cases both average weight and overweight. It can predict CRC at a cutoff 2633.4ng/ml with 82.3% sensitivity and 84% specificity. No specific Single Nucleotide Polymor-phism (SNP) in Insulin-like Growth Factor Binding Protein (IGFBP) gene could be associated with CRC.
Univariate logistic regression showed that CRC signifi-cantly increases by 5.74 for each one unit of IGFBP-3.
Conclusion: BMI and IGFBP-3 could be considered as effect modifiers for CRC risk. IGF-1 SNP rs6214 (TT and CT) was significantly associated with colorectal cancer risk regardless of the BMI.