A Comparative Study between Antioxidant Activity of Medical Ozone and Selenium on Experimentally Induced Diabetes in Male Rats
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- Category: Vol. 84, December 2016
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A Comparative Study between Antioxidant Activity of Medical Ozone and Selenium on Experimentally Induced Diabetes in Male Rats, AHMED T. MAHMOUD, MOHAMMED A. OKASHA, AYMAN S. SOLIMAN and MADEHA A. EWAIS
Abstract
Introduction: Ozone therapy has been used in recent decades and has been found useful in treatment of diabetes. It improves glycemia, prevents oxidative stress, and normalizes level of organic peroxides. Selenium is an essential dietary trace element that has an antioxidant effect. It functions as an integral part of glutathione peroxidase in addition to its important role in vitamin E metabolism and normal pancreatic functions.
Aim of Work: To determine the roles of ozone administra-tion in amelioration of oxidative stress in STZ-induced diabetes so as to establish its potential use in the strategy for treatment of diabetes.
Material and Methods: This study was applied on 50 male albino rats that were divided into 5 equal groups: Control group, diabetic group, ozone-treated diabetic group (received three IP injections/week on alternating days of medical ozone at a dose of 1.2mg/kg BW), selenium-treated diabetic group (received oral selenium as sodium selenite at a dose of 1.5mg/kg BW) and ozone plus selenium-treated diabetic group (received both ozone and selenium in the same previous schedule). The study proceeded for 4 weeks then fasting blood glucose and albumin concentrations were measured. MDA, glutathione, SOD, catalase, glutathione peroxidase, and glu-tathione reductase were measured in liver tissue homogenate. In addition, a histopathological study of the pancreas was performed.
Results: There were a significant increase in liver catalase, SOD, glutathione, glutathione peroxidase, glutathione reductase and a significant decrease in blood glucose and liver MDA in rats received medical ozone and/or selenium when compared to the diabetic group. The study also demonstrated that medical ozone and selenium had a synergistic antioxidant action. The histopathological study of the pancreas showed less necrosis and vaculations in rats received medical ozone and/or selenium when compared to the diabetic group.
Conclusion: The study confirmed the involvement of oxidative stress in DM and the ability of medical ozone similarly like selenium to up-regulate the activities of antiox-idant enzymes. Therefore, they could offer protection against oxidative damage in DM. Medical ozone and/or selenium could be used as an adjuvant therapy in control of diabetes and its complications.