Phenotypic and Genotypic Detection of Klebsiella Pneumoniae Carbapenemase,
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- Category: Vol. 84, March 2016
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Phenotypic and Genotypic Detection of Klebsiella Pneumoniae Carbapenemase, MAHA M. KOTB, NAGWA A. SEDKY, EMAN A. EL-SEIDI, ALAA R. AWAD and SAHAR S. AL-WAKEEL
Abstract
Aim: This study aimed to detect the presence of Klebsiella Pneumoniae Carbapenemase (KPC) enzyme among Entero-bacteriaceae by phenotypic tests; the Modified Hodge Test (MHT) and boronic acid inhibition test and to compare both methods to genotypic detection of blaKPC gene by Polymerase Chain Reaction (PCR).
Methodology: This study involved detection of KPC among 72 carbapenem non-susceptible isolates of enterobac-teriaceae by phenotypic methods as MHT and boronic acid inhibiton test followed by genotypic methods as PCR.
Results: The results of this study showed that the preva-lence of carbapenemase producing isolates by MHT was 51% and prevalence of KPC by carbapenem-boronic acid combined disk test was 35%, while it was 49% by genotypic method.
Conclusions: The presence of Klebsiella Pneumoniae Carbapenemase among enterobacteriaceae isolates causing healthcare-associated infections in the current study emphasizes the necessity for early detection of these isolates by phenotypic tests and genotypic tests.