ER-and ER-Expression in Thyroid Gland Lesions (Immunohistochemical and Histopathological Study)
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- Category: Vol. 85, September 2017
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ER-and ER-Expression in Thyroid Gland Lesions (Immunohistochemical and Histopathological Study), MIRAN A. EL-KORDY, FAHIMA M. METWALY, SAHAR M. TALAAT and AMIRA B. EL-ZAHED
Abstract
Background: Thyroid diseases (clinical and subclinical) are estimated to affect 10% of the population and they are more prevalent in females. This suggest a role of estrogen in the pathogenesis of thyroid diseases.
Objective: To evaluate the presence of estrogen receptors alpha and beta in different thyroid lesions and to detect if there is benign-malignant differences in their expression.
Material and Methods: 60 previously diagnosed specimens of various thyroid lesions were reviewed in this retrospective and immunohistochemical study. All were obtained from Pathology Department, Faculty of Medicine, Cairo University between January 2013 and December 2015. Formalin-fixed Paraffin-embedded tissue sections were used. Three sections were prepared from each paraffin block; one of them stained with Hematoxylin and Eosin (H & E) for histological re-evaluation, while others were subjected to the two immuno-histochemical markers (ER-a and ER-b). The (Allred) score method was used to evaluate immunohistochemical staining. The cases were considered positive if their score was more than 2, and showing nuclear immunoreactivity.
Results: Cases were 52 females and 8 males ranging in age from 17 to 72 years with mean 42.1±10.88 years. They were as follows: 8 cases (13.3%) non neoplastic, 11 cases (18.3%) benign, and 41 cases (68.3%) malignant. The ER-a positive cases were four cases (4/60; 6.7%) with non significant statistical correlations between ER-a immunostaining and patient age, sex, menopausal state or pathological diagnoses. The ER-b positive cases were 51/60 cases with significant correlation between both ER-b positivity and Allred scoring and different malignant pathological diagnoses with p-value (<0.05) for both. The expression is observed more in non neoplastic cases followed by malignant and at last benign cases. On contrary there were no significant correlation between ER-b expression and age, sex or menopausal status. On studying the expression of both ER-a and ER-b simulta-neously, we revealed that all positive ER-a cases were positive for ER-b but not the reverse but by using chi square, p-value was 0.384 which was statistically not significant.
Conclusion: ER-b is the more detectable estrogen receptor in thyroid tissue different lesions than ER-a and with more expression in non neoplastic cases followed by malignant cases.