Peripheral Blood DNA Methylation Markers (ALX4 and SEPT 9) For the Early Detection of Colorectal Carcinoma in the Egyptian Population: A Multicenter Study
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- Category: Vol. 85, September 2017
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Peripheral Blood DNA Methylation Markers (ALX4 and SEPT 9) For the Early Detection of Colorectal Carcinoma in the Egyptian Population: A Multicenter Study, ASHRAF O. ABD EL-AZIZ, HANY M. SHEHAB, RANIA M. LITHY, AYMAN R. AMER and DINA S. ABD EL-FATTAH
Abstract
Background: DNA methylation is one of the most impor-tant epigenetic mechanisms, which denotes the addition of a methyl group to DNA. Aberrant promoter demethylation is usually associated with overexpression of genes that might participate in pathogenesis of many diseases.
Aim of Work: To evaluate serum ALX4 and SEPT9 and their candidacy as novel noninvasive markers, in CRC among Egyptian patients.
Patients and Methods: A whole blood sample (10cc) will be collected in an EDTA-containing vacutainer tube and stored at 4C for a maximum of 24 hours. Samples will be delivered within this period to the molecular biochemistry lab for removal of the Buffy coat, DNA extraction and analysis. The molecular biologist will assess all samples in a blinded fashion.
Results: ALX4 with sensitivity (97.6%) and specificity (99.3%) with cut-off of 0.315μg/L which was higher than SEPT9 (sensitivity=87.4% and specificity=98.5%) with cut-off of 0.461μg/L.
Concluson: ALX4 and SEPT9 can be useful in high prediction of colorectal carcinoma with high sensitivity and specificity.