Vol. 85, December 2017

Efficacy of Laser Puncture on Neutrophils and T-Lymphocytes in Burned Patient

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Efficacy of Laser Puncture on Neutrophils and T-Lymphocytes in Burned Patient, WALAA A. ABD EL-AZIZ, MOHAMED M. KHALAF, AHMED M. YOUSEF and MAHA A. HASSAN

 

Abstract
Purpose: The current study was conducted to examine the effect of laser acupuncture on neutrophils and T-Lymphocytes in patient with burn injury.
Subjects and Methods: Thirty burned patients with burned surface area about 20% to 40% participated in this study. Their ages ranged from 25-40 years. They were selected from Orabi Hospital and were divided randomly into two equal groups. Group (A) composed of 15 patients who received traditional physical therapy program and medical treatment. Group (B) composed of 15 patients who received laser acu-puncture (infrared diode laser, wavelength 808 nanometer, Pulse duration: 200 nanosecond, power density: 0.4 Watt/cm2, energy density 4 Joule/cm2) which was placed in contact with the skin perpendicular over the body acupoints LU 1, LI 4, LI 11, GV 14, K 27, B 36 in both sides, with duration of 90 sec for each point three times per week for a month beginning after five days from burn injury in addition to traditional physical therapy program and medical treatment. Method of evaluation was measurements of neutrophils count by KX-2IN Hematology Analyzer, CD4 count and CD4/CD8 ratio by FACS Caliber flow cytometry device.
Results: There was a significant increase in neutrophils count, CD4 count and CD4/CD8 ratio in laser acupuncture group when compared with control group.
Conclusion: Laser acupuncture can be considered as an effective method in increasing neutrophils and T-lymphocytes count post burn.

 

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