Genetic Association of Matrix Metalloproteinase MMP-1, MMP-3 and MMP-9 Genes with HCVRelated Hepatocellular Carcinoma in Egyptian Patients
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- Category: Vol. 85, December 2017
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Genetic Association of Matrix Metalloproteinase MMP-1, MMP-3 and MMP-9 Genes with HCVRelated Hepatocellular Carcinoma in Egyptian Patients, BELAL A.E. MONTASER, WALEED M. FATHY, NAGLAA S. EL-ABD, SAFAA I. TAYEL and AL-SHIMAA M. AL-HANAFY
Abstract
Background and Aim: Matrix Mctalloprotcinascs (MMPs) are multifunctional proteins that assume an urgent part in cell development, differentiation, inflammation and angiogenesis. MMPs gene polymorphism might be included in movement of HCV related Hepatocellular Carcinoma (HCC). The study expected to explore relationship of ММР-1-1607 10120, ММР-3-1171 5A/6A and ММР-9-1562 Cam' genes polymorphisms with liver cirrhosis and HCC patients.
Methods: The study enrolled 50 patients with liver cirrhosis, 48 patients with НСС on top of liver cirrhosis and 30 healthy controls. Alphafetoprotein (AFP), CHILD score, abdominal ultrasonography and triphasic СT were assessed. Genes polymorphisms were made by PCR-RFLP.
Resuits: НСС patients had higher mutant 0202(35.4%) and 02 allele (625%) of MMP- I gene than both cirrhotic (p <0.05) and control groups (p<0.001) and furthermore cirrhotic patient than control group (p<0.05). For ММР-3 gene, additionally, НСС patients had the most noteworthy predominance of mutant 5A/5А (22.9%) and 5A allele (52.1%) than both cirrhotic (p<0.05) and control groups (p<0.001) and also higher in cinhotic patients than control group (р<0.05). The results of МMР-9 gene uncovered higher frequency of mutant TT genotype and T allele in both НСС (56.3% and 74% respectively) and cirrhotic groups (10% and 35% respectively) than control group. Likewise, critical relationship of AMP genes with CHILD score, tumor size and staging of HCC.
Conclusion: AMP-1,3, 9 gene mutation may be embroiled in progression of liver cirrhosis and hazard relationship for НСС development.