Serum Obestatin Level in Letrozole-Induced PCOS Rat Models: Correlation with Some Inflammatory and Oxidative Parameters
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- Category: Vol. 85, December 2017
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Serum Obestatin Level in Letrozole-Induced PCOS Rat Models: Correlation with Some Inflammatory and Oxidative Parameters, MARWA A. HABIB and MOUSTAFA H. ABD EL-SALAM
Abstract
Background: Polycystic Ovarian Syndrome (PCOS) is a common endocrinopathy that cause anovulatory infertility where oxidative stress and inflammation are involved in its pathogenesis. Obestatin is an anorexigenic peptide derived from proghrclin and has anti-inflammatory and anti-oxidative properties.
Aim of the Work: То assess serum levels of obestatin in letrozole-induced PCOS rat model and to evaluate its relation to insulin resistance, sex hormones, some inflammatory and oxidative stress markers.
Material and Methods: 48 virgin female albino rats were divided into two equal groups; group I (ordinary diet) and group II (high fat diet) for 9 weeks, then each group was subdivided into subgroup A (control, water given daily for 3 weeks) and subgroup В (PCOS induced, letrozole given daily for 3 weeks) and remained on their diets. Serum levels of obestatin, testosterone, estradiol, progesterone, III, FSII, insulin, glucose and TNF-a were estimated, and ovaries were dissected and used for histopathological examination and evaluating MDA, SOD and GPx levels.
Results: Persistent estrus, increased testosterone levels, and multiple large ovarian cystic follicles were detected in PCOS groups. Serum obestatin levels were decreased in Letrozole-induced PCOS in lean and obese rats. Serum obestatin levels negatively correlated with BM[, HOMA-IR, serum insulin, glucose, TNF-a, ovarian MDA, SOD and GPx, but were not correlated with the levels of sex hormones in all studied groups.
Conclusion: The decreased serum obestatin levels in PCOS can be partly attributed to hyperinsulinemia and may give an explanation for the inflammatory and oxidative state detected in PCOS.