Effect of Obestatin on Experimentally-Induced Chron's Disease in Rats
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- Category: Vol. 85, June 2017
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Effect of Obestatin on Experimentally-Induced Chron's Disease in Rats, NANEES F. EL-MALKEY and DOAA A. ABD EL-MOETY
Abstract
Background: Crohn's Disease (CD) is an inflammatory disease manifested by ulceration and digestive disturbances commonly in upper gastro-intestinal tract. Previous studies have shown that Obestatin (OB) exhibits protective and regenerative effects on stomach. However, little is known about the effect of obestatin administration on small intestinal inflammation.
Aim: To evaluate the anti-inflammatory and anti-oxidant effect of obestatin on intestinal injury in a rat model of of Crohn's disease.
Methods: 30 animals were divided into three equal groups (n=10); Group I: (healthy control), Group II (CD group): In which rats received two subcutaneous injections of indometh-acin one day a part for chronic Chron's disease induction, and Group III (OB-treated group): Obestatin (8nmol/kg/dose) was administered intraperitoneally twice a day, starting on day four after indomethacin treatment. The effect of indomethacin and obestatin treatment were assessed on day seven by mor-phological, histological, and biochemical analysis of intestinal interleukin-1b (IL-1B), Myeloperodidase (MPO), Malondial-dehyde (MDA), Catalase (CAT) activity, glutathione peroxidase (GPx) and Superoxide Dismutase (SOD).
Results: Indomethacin administration resulted in Chron's disease evidenced by morphological and histological changes of intestinal injury accompanied by significant increase in IL-1B, MPO, MDA levels; with concomitant decreases in antioxidant enzymes. However, indomethacin-induced intes-tinal damage and oxidative injury were ameliorated in the obestatin-treated group.
Conclusion: The present study revealed that administration of obestatin maintains a balance in oxidant-antioxidant status through the increase in endogenous antioxidants and the decrease in pro-inflammatory mediators in rat model of Chron's disease.