Study on Matrix Metalloproteinases and their Inhibitors in Breast Cancer
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- Category: Vol. 85, June 2017
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Study on Matrix Metalloproteinases and their Inhibitors in Breast Cancer, MONA SULTAN, MARVAT S. MOHAMED, MOHAMED ALI and MAHMOUD MOAWAD
Abstract
Background: MMP2 (matrix metallopeptidase 2), this gene is a member of the Matrix Metalloproteinase (MMP) gene family, that are zinc-dependent enzymes capable of cleaving components of the extracellular matrix and molecules involved in signal transduction. The reversion-inducing cys-teine-rich protein with Kazal motifs (RECK) gene was initially isolated as a transformation suppressor gene.
Objective: The objective of this study was to characterize the relationship between RECK and MMP-2 expression and the clinical manifestation of invasive breast cancer.
Subjects: Immunohistochemistry and Reverse Transcrip-tion-Polymerase Chain Reaction (RT-PCR) were employed to detect the protein of some prognostic factors and mRNA expression of RECK and MMP-2 in invasive breast cancer.
Results: The RECK gene amplification, by RT-PCR was positive in 20% while MMP2 gene amplification was positive in 30% of invasive duct breast cancer and showed the MMP2 gene was significantly associated with bad prognostic param-eters as lymph nodes involvement and absence of Estrogen and progesterone receptors. RECK gene was significantly associated with favorable prognostic factors as absence of lymph nodes involvement and presence of Estrogen and progesterone receptors. In conclusion: RECK and MMP2 genes are a significant prognostic factor correlated with invasive breast cancer.
Conclusions: RECK and MMP2 genes expression is therefore a potentially useful prognostic marker for breast cancer.