A Study on Serum Obestatin Level in Experimentally-Induced Polycystic Ovary Syndrome in Lean and Obese Rats
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- Category: Vol. 85, June 2017
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A Study on Serum Obestatin Level in Experimentally-Induced Polycystic Ovary Syndrome in Lean and Obese Rats, DOAA A. ABDEL MOETY
Abstract
Background: Obestatin is an anorexic hormone produced in cells of gastric mucosa and plays an important role in regulation of body weight. Also, it is closely related to insulin resistance (IR), and other metabolic parameters. Polycystic ovary syndrome (PCOS) is a common endocrine disorder affecting females in the reproductive period and is associated usually with obesity, IR, and cardiovascular risk factors. Due to presence of similar relationships between each of obestatin and PCOS with each of obesity, IR, and other metabolic parameters, few researches have studied the link between serum obestatin levels and PCOS.
Aim: To estimate serum obestatin level and, to study its relationships to body mass index (BMI), IR and lipid profile in PCOS rats.
Material and Methods: 30 young virgin healthy female of local strain albino rats were used. Rats were divided into three equal groups: Group I (control), group II (lean PCOS) rats fed on commercial rat standard chow for 9 weeks and then received a daily single dose of letrozole orally (0.5mg/kg) for 21 days. Group III (obese PCOS) rats fed on high fat diet for 9 weeks, and then received a daily single dose of letrozole (0.5mg/kg) orally for 21 consecutive days. At the end of experiment, serum levels of testosterone, estradiol, progest-erone, luteinizing hormone (LH), follicular stimulating hor-mone (FSH), obestatin, glucose, insulin were measured. BMI and HOMA-IR were calculated. Serum cholesterol, triglycer-ides (TG), low (LDL) and high (HDL) density lipoproteins were also evaluated. Ovarian histopathology was done.
Results: Obese PCOS rats showed significant reduction in serum obestatin levels accompanied by significant hyper-glycemia, hyperinsulinemia, IR, and hyperlipidemia. However, lean PCOS rats showed no changes in serum obestatin levels nor any of the estimated metabolic parameters when both compared to control group.
Conclusion: Serum obestatin levels were negatively correlated with BMI, hyperglycemia, hyperinsulinemia, IR, hyperlipidemia in obese PCOS rats, and this may be related to obesity and other associated metabolic parameters rather than to PCOS itself.