Serum Chemerin Levels in Normal Pregnant and Gestational Diabetic Albino Rat Model,
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- Category: Vol. 85, June 2017
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Serum Chemerin Levels in Normal Pregnant and Gestational Diabetic Albino Rat Model, NADINE A. RAAFAT
Abstract
Background: Gestational diabetes mellitus (GDM) is currently gestational disorder which associated with insulin resistance. Chemerin is an adipocytokine linked to inflamma-tion and obesity. Data proposed chemerin levels in normal pregnancy and GDM are still controversial.
Objective: To assess serum chemerin levels in final stage of pregnancy and to examine the influence of experimental induced gestational diabetes on chemerin levels in albino rats. Also, to explore the association between chemerin levels, certain metabolic and inflammatory parameters in normal pregnancy and GDM.
Material and Methods: Healthy virgin female albino rats weighing 100-130 gm were divided randomly into three groups (n=12 rats): Group I (Control) group; virgin rats were fed normal diet. Group II (normal pregnant) group; rats were fed normal diet for five weeks before induction of pregnancy. Group III (GDM-induced group); rats were fed Fatty-sucrose diet (FSD) for five weeks before induction of pregnancy, then they were injected intraperitoneally (I.P) by streptozotocin (STZ) (25mg/kg) on the 7th day of gestation. For all groups; body mass index (BMI), serum estradiol, progesterone, chemer-in, Total triglycerides (TG), total cholesterol (TC), low density lipoproteins-cholesterol (LDL-c), high density lipoproteins-cholesterol (HDL-c), C-reactive protein (CRP), glucose, insulin levels and calculated homeostatic model assessment of insulin resistance index (HOMA-IR) were estimated.
Results: Normal pregnant rats showed a significant in-crease in BMI, serum chemerin, CRP, TC, TG, LDL-c, insulin levels and HOMA-IR compared to controls. Additionally, serum chemerin levels were significantly and positively correlated with BMI, serum glucose, insulin, CRP, TC, TG levels and HOMA-IR. GDM-induced rats had significantly increased levels of serum chemerin, CRP, TC, TG, LDL-c, glucose and HOMA-IR, while decreased insulin and HDL-c levels compared to normal pregnant and control rats. Moreover, serum chemerin levels showed significant positive correlations with BMI, HOMA, glucose, TG, TC, LDL and CRP levels and an inverse correlation with HDL-c levels in GDM-induced group.
Conclusion: Late pregnancy is accompanying with rising of serum chemerin levels to favor a pro-inflammatory condi-tion. Moreover; serum chemerin levels are greatly increased in gestational diabetes possibly for compensating GDM-induced insulin resistance, dyslipidemia and inflammatory response.