miRNA Expression Profiling in Pediatric B-Cell Acute Lymphoblastic Leukemia by Microarray Technology
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- Category: Vol. 86, March 2018
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miRNA Expression Profiling in Pediatric B-Cell Acute Lymphoblastic Leukemia by Microarray Technology, EMAN EL-SHARAWY, MOHAMED EL-SHANSHORY, SAID ABDOU, LAILA M. SHREIF, ABDEL-AZIZ A. ZIDAN and ENAS A.A. BAKI
Abstract
Background: MicroRNAs (miRNAs) are endogenous non-coding RNAs, which play an essential role in the regulation of gene expression.
Aim of the work: To analyze miRNA expression profile in the childhood B acute lymphoblastic leukemia (B-ALL). To understand the functional regulatory effects of differential miRNAs on their targets and its clinical significance and novel analytical method to combine their expression profiles.
Patients and Methods: In this study, we determined the relative expression analysis of miRNA and mRNA profiles in 20 childrenB-ALL patients. Through the use of miRNA microarray technology and according to their miRNA pro-file.The best characterized non-coding RNA family consists in humans of about 1400 microRNAs for which abundant evidence have demonstrated fundamental importance in normal development, differentiation, growth control and inhuman diseases such as cancer.
Results: miR-100, miR-146a, miR-128a, miR-128b, miR-181a, miR-34, miR-193a, miR-193b, miR-151, miR-130b, and miR-125b were found to be the most consistently upreg-ulated miRNAs in B-acute lymphoblastic leukemia patients as compared with healthy control.
Conclusion: To analyze miRNA in B-ALL children patients before and after induction of chemotherapy and measure the correlation of these results using microarray technique.
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