Determination of Vitamin D3 Content in High, Low and Zero Fat Food Using High Performance Liquid Chromatography
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- Category: Vol. 86, December 2018
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Determination of Vitamin D3 Content in High, Low and Zero Fat Food Using High Performance Liquid Chromatography, AHMAD M. FARAG, MAHMOUD S. RIZK, HAMDY A. EL-BASSEL and MONA H. YOUSSIF
Abstract
Background: Vitamin D, has a significant role in bone metabolism and helps calcium absorption in the body. There are only few vitamin D assay methods available for zero fat food.
Aims of Study: (1) To develop an accurate and sensitive LC method for the quantification of vitamin D3 in food by
optimization of each step of the analytical method: Extraction, sample preparation, separation and detection. (2) To validate the developed method. (3) To apply the method to quantify the total vitamin D3 in food from several species.
Material and Methods: In this study, a rapid, simple, and economical reversed phase liquid chromatographic method was described for the determination of vitamin D3 , in some high, low and zero fat samples (milk products, cereal and chewing gum samples). The isolation of fat soluble vitamins includes a saponification step and an extraction step with petroleum ether and diethyl ether. The vitamin D3 content of samples was determined by reversed phase liquid chromatography.
Ultra violet-Visible (UV-VIS) detector and C18 column were used for this purpose.
Results: The linearity of standard curves of vitamin D3 were 10-200 mg/ml expressed of the correlation coefficient r2 = 0.9992). The detection (LOD) and quantification (LOQ) limits were 5.09 and 15.42mg/ml, respectively. The accuracy was 101.37±4.37.
Conclusion: The described reversed-phase HPLC method is favorable compared with other published HPLC-UV methods (20 and 21) because of its stability-indicating nature, short run time and wide analytical range with outstanding linearity, accuracy and precision. The proposed method allows the determination of vitamin D3 in a single chromatographic run and is suitable for the analysis of the stability of vitamin D3 .
The obtained results from the assay of vitamin D3 in commercial nutrition supplements confirmed that the method is appropriate for the routine analysis of various food samples.