Histological Study of the Effect of Gentamicin on Kidneys of Rats, HANAA A. IBRAHEM, SAFINAZ S. SAYED, SAMEH F. EL SONBATY and HEBA A.A. MAHMOUD
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- Category: Vol. 87, June 2019
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Histological Study of the Effect of Gentamicin on Kidneys of Rats, HANAA A. IBRAHEM, SAFINAZ S. SAYED, SAMEH F. EL SONBATY and HEBA A.A. MAHMOUD
Abstract
Background: Gentamicin is one of the most important aminoglycoside antibiotics used widely for the treatment of serious and life-threatening infections, however its clinical utility is limited by the frequent incidence of acute renal failure.
Aim of Study: This study was designed to detect the histological and ultrastructural changes of Gentamicin on kidney.
Subjects and Methods: Biopsies were obtained from right kidney of 10 male albino rats and 10 normal control. They were processed and stained with hematoxylin & eosin stain. Other biopsies from left kidney were prepared for transmission electron microscopies. This was followed by morphometric measurements and statistical analysis of the results and measurements of urea and creatinine in blood for rats.
Results: Light microscopic examination of the kidney sections showed marked tubular damage especially at PCT in Gentamicin treated group. These histological changes were detected by histochemical stains, image analyzer as well as TEM and also proved by measurement of urea and creatinine in blood. Described morphological, biochemical and functional changes in kidney due to gentamicin may be due to ROS that cause nephrotoxicity.