Value of Vitamin D Administration Versus Suppressing Gut Microbiota in Modifying Metabolic Associated Osteoarthritis in Rats
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- Category: Vol. 88, March 2020
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Value of Vitamin D Administration Versus Suppressing Gut Microbiota in Modifying Metabolic Associated Osteoarthritis in Rats, RANDA S. GOMAA, JEHAN SAEED and SAFYA E. ESMAEEL
Abstract
Background: Metabolic syndrome (MetS) is considered as common risk factors for knee osteoarthritis (OA). Aim of Study: Comparing the effect of vit. D supplemen-tation and gut microbiota suppression on the development of metabolic knee OA in rats is the aim of the study. Material and Methods: Forty adult male rats were subdi-vided into control and high carbohydrate high fat (HCHF) fed rats that furtherly subdivided into: HCHF (positive control), HCHF-Vitamin D treated & HCHF-antibiotics treated. Body mass index (BMI), serum glucose, insulin, total cholesterol, triglycerides, HDL-C, LDL-C, inflammatory cytokines were estimated. Homeostatic model assessment for insulin resistance (HOMA-IR) was calculated. Synovial cytokine, tissue degen-erative enzymes gene expression and knee histopathology were assessed. Results: HCHF induced significant increase in BMI, glucose, insulin, HOMA-IR, serum and synovial cytokines and degenerative enzymes with dyslipidemia and knee joint damage compared with controls. Vit. D improved all parameters in comparison to HCHF fed group. Antibiotic administration improved glucose, insulin, HOMA-IR, dyslipidemia and serum cytokines but induced insignificant changes in BMI, synovial cytokines, degenerative enzymes and knee joint damage compared with HCHF fed group. Conclusion: HCHF diet led to development of knee joint damage associated with changes in the metabolic profile in rats. Vit D supplementation dramatically improved knee joint damage, obesity insulin resistance, dyslipidemia and inflam-mation systemically and locally while controlling gut dysbiosis by antibiotics did not improve obesity, local inflammation and knee damage but improved the other parameters less than Vit D.