Parasitological Studies on Trichomonus Vaginalis on Female Patients Presented with Vaginal Discharge at Aswan University Hospital
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- Category: Vol. 89, June 2021
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Parasitological Studies on Trichomonus Vaginalis on Female Patients Presented with Vaginal Discharge at Aswan University Hospital, AHMED K. DYAB, HANY A. FAROUK, MOHAMMED F. MOHAMMED and TASNEEM M. HASSAN
Abstract
Background: Trichomonas vaginalis (T.vaginalis) is one of the most common parasitic non viral Sexually Transmitted Infections (STI) in the world. The World Health Organization (WHO) has estimated that 160 million trichomoniasis cases are acquired annually worldwide [1]. Aim of Study: Evaluate different direct laboratory methods used in diagnosis of vaginal trichomoniasis. Material and Methods: The cross sectional study was carried out on 200 female patients, suffering from vaginal discharge, aged between 20-50 years old. Two vaginal swabs were taken from every patient after taking a concent. All the samples were examined by wet mount, stained by Giemsa, rapid test and Culture on modified Diamond's medium and ELIZA. Results: This study showed that ELIZA is the gold stan-dered among diagnostic techniques as it detected T. vaginalis in 15% of suspected patients followed by 13.5% by culture, then 12.5% by rapid test and lastly wet mount which detected only 5% of cases. ELIZA and Culture gave the highest sensi-tivity, specificity, accuracy, NPV and PPV followed by rapid test. The least sensitive was wet mount. The least specific was Giemsa stain. Conclusion: The study showed that wet mount method is simple and cheap. Wet mount microscopy is the preferred option for prompt diagnosis of trichomoniasis. It is proved that rapid test is relatively simple to carry out, shows reasonable sensitivity and culture remains the most accurate single method for detecting the presence of T. vaginalis in patient samples. and Diamond's medium is a suitable culture method and is considered as the gold standard of diagnosis of trichomoniasis.