Prevalence of Ochrobactrum Intermedium in Gastric Biopsy Specimens of Patients with Gastritis and Dyspepsia and the Impact of Amniotic Membrane as Antibacterial
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- Category: Vol. 92, December 2024
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Prevalence of Ochrobactrum Intermedium in Gastric Biopsy Specimens of Patients with Gastritis and Dyspepsia and the Impact of Amniotic Membrane as Antibacterial, WAFAA ELSHABSHIRY, RATEB ABBAS, ELHAM AWAD, MEDHAT HASHEM, ASHRAF EID, AHMED SEIF and OMAIMA KHAMISS
Abstract Background: Ochrobactrum intermedium bacteria has some roles as an opportunistic organism in many cases of gas-tritis and dyspepsia. Human amniotic membrane is known to have an antimicrobial effect on several organisms. Aim of Study: This study briefly aims to screening for a novel anti-bacterial treatment on some pathogenic gastrointes-tinal tract bacteria. Material and Methods: Sixty-three gastric biopsies from dyspeptic patients were collectedfrom (Shebin El-Kom Teach-ing Hospital, Shebin El-Kom, Egypt). Selected isolate of O.in-termedium from thirty positive isolates was molecular identi-fied and phylogenic tree was carried out using 16S rRNA which was sequenced and compared with available 16S ribosomal se-quences in the NCBI GenBank database (OR067155). Human Amniotic membrane (HAM) was freshly isolated from the pla-centa and HAECs have been enzymatically isolated using pre-warm 0.05% trypsin/EDTA, then cells were seeded in standard culture medium DMEM/F12 (Gibco, Thermo Fisher Scientific, USA) supplemented with 10% FBS and incubated at 37ºC. Cell counts was done using a hemocytometer, cell maintenance and viability was followed-up using trypan blue. Possible inhibi-tory activity of HAM against O.intermedium has been tested and recorded. Ochrobactrum genus have been confirmed in the human gastric niche based on PCR. Results: Biopsies from patients with gastroesophageal re-flux disease showed H.pylori existence in histopathology with also positive urease, catalase and oxidase tests, while PCR re-sults showed O.intermedium without H.pylori. Human amniot-ic membrane had an inhibitory effect against O.intermedium. Conclusion: This study demonstrates that Human amniotic membrane can be used as an antibacterial against O.intermedi-um in cases of gastritis or dyspepsia, especially in immunocom-promised patients, O.intermedium should be considered as an opportunistic organism whether H.pylori exists or not.