Immunohistochemical Cyclooxygenase-2 (COX-2) and P53 Expression in Breast Carcinoma with Correlation to Clinico-Pathological Parameters
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- Category: Vol. 81, March 2013
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Immunohistochemical Cyclooxygenase-2 (COX-2) and P53 Expression in Breast Carcinoma with Correlation to Clinico-Pathological Parameters,EMAN M.S. MUHAMMAD, HALA SALAH EDIN, MARCELLE N. GUIRGUIS and SAMY M. OSMAN
Abstract
Background/Aims: Breast cancer is the most common cancer in Egyptian women.
This Study Aims to:
1-Evaluate COX-2 and p53 expressions in the successive steps of breast carcinogenesis.
2-Determine the correlation between COX-2 and p53 with the clinico-pathological parameters in ductal breast carci-noma.
Patients and Methods: This study included 74 specimens of breast lesions. Data about patient's age, tumor size and local aggressive changes, history of recurrence and/or presence of distant metastasis were obtained from clinical sheets. Hematoxylin and Eosin (H&E) stained sections were evaluated for histopathological tumor type, tumor grade, presence or absence of normal, hyperplastic, in situ component, lympho-cytic infiltration, lymphovascular invasion, and axillary lymph node status. COX-2 and p53 immunostaining was done to detect their expressions using the avidin-biotin peroxidase method.
Results: COX-2 and p53 expressions increased with increasing grade of ductal carcinoma in situ (DCIS) and invasive ductal carcinomas (IDC) (p<0.05 and p<0.002 re-spectively for COX-2) and (p<0.01 andp<0.002 respectively for p53). COX-2 and p53 expressions increased progressively along the continuum of neoplastic changes from normal breast epithelium to IDC (p<0.01 for each). There was significant correlation between either COX-2 or p53 and tumor size (p<0.05 for each), tumor grade (p<0.002 for each), lymphovas-cular invasion (p<0.03 & <0.02 respectively). There was significant correlation between COX-2 and lymph node me-tastasis (p<0.02). There was significant correlation between p53 and lymphocytic infiltration (p<0.03). There were positive correlations between COX-2 and p53 in DCIS and in IDC (p<0.000 for each).
Conclusion: Both COX-2 and p53 were increased with poor prognostic parameters; tumor size, tumor grade, lym-phovascular invasion, lymph node metastasis and lymphocytic infiltration. P53 is likely to be involved in the regulation of COX-2 expression in ductal breast cancer. These findings might imply for new therapeutic strategies in order to prevent progression of DCIS to IDC and to improve breast cancer therapy.