CDH4 and CDKN2B/P15 for the Detection of Colon Cancer in the Egyptians
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- Category: Vol. 85, September 2017
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CDH4 and CDKN2B/P15 for the Detection of Colon Cancer in the Egyptians, ASHRAF O. ABDELAZIZ, HANY M. SHEHAB, RANIA M. LITHY, DINA S. ABDEL FATTAH and AYMAN R. AMER
Abstract
Background: Colorectal cancer (CRC), including anal, is the 3rd most common cancer in the world. It is the 4th most common cause of cancer death worldwide. DNA methylation in gene promoter regions often results in long-term silencing of gene expression. Meanwhile, aberrant promoter demethy-lation is usually associated with overexpression of genes that might participate in pathogenesis of many diseases.
Aim of Work: To evaluate serum CDH4 and CDKN2B/P15 and their accuracy as novel noninvasive markers for the detection of CRC among Egyptians.
Patients and Methods: This is a cross sectional study and it was performed on 292 patients presenting for a colonoscopy at the Gastrointestinal Endoscopy Unit in Kasr El-Ainy Hospital, Cairo University and National Cancer Institute from January 2014 to June 2015. Whole blood sample was subjected to whole blood DNA extraction and analysis. The molecular biologist will assess all samples in a blinded fashion.
Results: CDKN2B/P15 shows higher (sensitivity=96.1% and specificity=97.8%) with cut-off of 0.4 12mg/L than CDH4 with sensitivity=75.6% and specificity=70.1% with cut-off of 0.330μg/L.
Concluson: CDH4 and CDKN2B/P15 may be highly sensitive and specific in prediction of colorectal cancer.