Clinicopathologic Significance of the Expression of SPOCK1 and HDAC1 in Urothelial Bladder Cancer; An Immunohistochemical Study
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- Category: Vol. 85, September 2017
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Clinicopathologic Significance of the Expression of SPOCK1 and HDAC1 in Urothelial Bladder Cancer; An Immunohistochemical Study, ABLA S. MAHMOUD, DINA A. KHAIRY, OLA A. HARB and MAGED M. ALI
Abstract
Background: Urinary Bladder Urothelial Carcinoma (UBUC) continued to be the commonest malignancy of the urinary bladder and because its incidence rate, invasion and spread were increasing despite improvement in therapeutic choices, it is needed to identify new biomarkers that has relation to pathogenesis of its spread and poor clinicopatho-logical parameters which can help to find new therapeutic targets for such type of cancer. Sparc-osteonectin-cwcv and kazallike regions (SPOCK1) proteoglycan 1 played an impor-tant role in cell-cycle control, DNA repair, apoptosis, invasion and metastasis. Histone deacetylases (HDACs) were derived from yeast (Saccharomyces cerevisiae) to humans and histone acetyltransferases. Human HDACs had been classified into 4 main types depending on the study of their composition and similarity to yeast enzymesa detailed immunohistochemical expression analysis of SPOCK1 and HDACs in urothelial carcinomas has not been studied in details to date.
Aim: To analyze the immunohistochemical expression patterns of SPOCK1 and Histone deacetylase 1 (HDAC-1) in Urinary Bladder Urothelial Carcinoma (UBUC) then correlated such expressions with its clinicopathological parameters.
Methods: SPOCK1 and HDAC-1 were evaluated using immunohistochemical expression in 50 paraffin blocks from 50 patients with UBUC. The relationship between their ex-pressions and clinicopathological parameters was analyzed.
Results: The expression of SPOCK1 in UBUC was strong-ly positively correlated with age of the patients (p=0.035), grade and stage of the tumor (p<0.001), distant metastasis, vascular invasion (p=0.03 1), perineural invasion (p=0.004), adjacent carcinoma in situ (p=0.041) and mitotic rate (p= 0.003). The expression of HDAC-1in UBUC was significantly positively correlated with grade and stage of the tumor, distant metastasis, vascular invasion, perineural invasion, growth pattern, multiplicity, mitotic rate (p<0.001) and size of the tumor (p=0.027).
We found a positive correlation between both SPOCK1 and HDAC-1expressions in UBUC (p=0.007).
Conclusion: SPOCK1 and HDAC-1expressions were significantly positively correlated with adverse clinicopatho-logical parameters of UBUC.