Assessment of miRNA-202 for Early Detection of Egyptian Women Breast Cancer
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- Category: Vol. 85, September 2017
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Assessment of miRNA-202 for Early Detection of Egyptian Women Breast Cancer, WALEED M. FATHY, SABAH FAROUK, GAMALAT EL-GEDAWY, SHAIMAA SOLIMAN, MOHAMED SABRY and SHIMAA FAHIM
Abstract
Background: Breast cancer is one of the most common cancers worldwide. It is considered as a heterogeneous disease comprising various types of neoplasms, which involves dif-ferent profile changes in both mRNA and miRNA expression. miRNAs are short non-coding regulatory RNAs of (20-25 nucleotides) that generally regulate gene expression at the post-transcriptional level. They are over expressed in many human cancers including Breast Cancer (BC), so can offer great potential as biomarkers for cancer detection due to their remarkable stability in blood and their characteristic expression in many different diseases. miRNA-202 is one of miRNAs that is up-regulated in breast cancer so, it could be a useful marker for diagnosis and prognosis of this cancer.
Objectives: To estimate the diagnostic potential of circu-lating miRNA-202 in Egyptian women with breast cancer in comparison to healthy controls.
Subjects and Methods: This study was conducted on 50 patients with Breast Cancer (BC), 25 patients with Benign Breast Disease (BBD) and 25 apparently healthy women as a control group. All participants were tested for circulating miRNA-202 by real-time quantitative Polymerase Chain Reaction (RT-qPCR) and CEA, CA15-3, CA27-29 by ELISA.
Results: miRNA-202 rises dramatically between BC cases than both BBD cases and healthy controls, as the result shows that miRNA-202 was significantly higher in BC group (p< 0.001) than both BBD and control groups. Receiver Operator of Characteristics (ROC) curve analysis of circulating miRNA-202 revealed that, at a cut-off value of 14.35 (fold expression), the sensitivity and specificity for differentiation of BC cases from non-cancer subjects were 96% and 98% respectively. A significant positive correlation between miRNA-202 RQ and each of CEA, CA 15.3, CA 27.29 were found.
Conclusion: Circulating miRNA-202 might be a biomarker for early diagnosis of breast cancer.