Single Nucleotide Polymorphisms of IL-10 and IL-18 Promoter Regions in Type 2 Diabetic Patients with Nephropathy
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- Category: Vol. 85, December 2017
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Single Nucleotide Polymorphisms of IL-10 and IL-18 Promoter Regions in Type 2 Diabetic Patients with Nephropathy, GIHAN A. SLEEM, HALA M. ALI, MANAL EL-DEEB and LAILA A. RASHED
Abstract
Background and Aim: Different inflammatory molecules including pro-inflammatory cytokЊes play an important role in the development of Diabetic Nephropathy (DN). hxterleuldn10 (IL-10) and IL-18 polymorphic variants are linked with cytokine production and are involved in type 2 diabetes mellitus. The purpose of this study is to analyze IL-10 (-592) and IL-18 (-607) gene polymorphisms, and to find their association with serum levels of IL- 10, IL-18 and its Binding Protein (ВР) in patients with diabetic nephropathy.
Subjects and Methods: This study included 180 subjects, divided into three groups, group 1: 40 healthy normal control subjects, group 2: 70 diabetic patients without nephropathy and group 3: 70 diabetic patients with nephropathy. The IL-10 (-592) and IL-18 (-607С/A) gene polymorphisms were detected by PCR]RPLP, while the serum levels of IL-10, IL-18 and IL-18ВР were determined by ELISA.
Results and Conclusion: There was significant increase in the frequency of IL-10 (-592) AА genotype, IL-18 (-607) CC genotype and its A allele, C allele respectively in diabetic nephropathy patients. Also, there was highly significant increase in IL-10. IL-18 and IL-18BP serum levels in ON group compared to diabetic group without nephropathy and to control group.
Key Words: Diabetic nephropathy - IL-10 - IL-18 - lL-18 Вр - Single nucleotide polymorphisms.