Evaluation of Antimicrobial Efficacy of Four Tested Solutions against Enterococcus Faecalis, an in Vitro Study
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- Category: Vol. 85, December 2017
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Evaluation of Antimicrobial Efficacy of Four Tested Solutions against Enterococcus Faecalis, an in Vitro Study, HAYAM Y. HASSAN, SAMIRA ZAKEER and NORA F. MAHMOUD
Abstract
Aim of Study: This in vitro study is to evaluate the anti-bacterial activity of four selected solutions (Biopure MTAD, 2% Nano-Chitosan, 2% Chitosan, 2.5% sodium hypochlorite) against Enterococcus faecalis (E. faecalis).
Methods: The antimicrobial efficacy of four tested solu-tions was primarily determined using the agar disk-diffusion method. Six inoculated Mueller-Hinton agar plates were prepared by spread of 100ml of freshly prepared culture over the surface of each agar plate. Then, filter paper discs of 6mm diameter were set and loaded with approximately 10ml of each tested solutions and placed onto the surface of each inoculated plate. All plates were incubated at 37 ºC for 48 hours and the zones of inhibition were recorded. Furthermore, a total of 50 standardized single-canaled human teeth were collected and sterilized. Five teeth were cultured directly as negative control. Others were infected by a trypticase soy broth inoculated with E. faecalis. Infected roots incubated at 37ºC for 48 hours. Then five teeth cultured without treatment as positive control. While, the remaining were randomly divided into four groups (n=10) for being tested using the four selected solutions. Two or three sterile paper points were inserted into every root canal then transferred aseptically into corresponding Wassermann tubes that contain sterile Trypticase soy broth. All Wassermann tubes were incubated at 37ºC for 48 hours then microbial growth of E. faecalis was verified by turbidity of the broth. Growth was further checked after 2 and 10 days then obtained data were statistically analysed.
Results: Of the two methods, Biopure MTAD significantly inhibited the bacteria growth recording 0% and the 2% Nano-Chitosan, 2% chitosan and 2.5% sodium hypochlorite recorded some negative and some positive results so there is significant difference between the tested solutions. At 10 days’ time interval the antimicrobial activity of all the tested solution generally decreased against E. faecalis.
Conclusion: Biopure MTAD was found to efficiently improve antimicrobial root canal disinfection than other tested solutions against E. faecalis (p<0.05).