Therapeutic Effect of Crocin in Collagen Induced Rheumatoid Arthritis Rat Model (Crocin in Rheumatoid Arthritis)
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- Category: Vol. 85, March 2017
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Therapeutic Effect of Crocin in Collagen Induced Rheumatoid Arthritis Rat Model (Crocin in Rheumatoid Arthritis), MARWA N. EMAM and RANIA N. ABD EL-LATIF
Abstract
Introduction: Rheumatoid arthritis (RA) is an autoimmune chronic inflammatory disease where articular cartilage degen-eration and inflammation are the hallmark of progressive arthritis and they represent the major causes of disability. Several medicinal plants and their isolated molecules have been used in modern medicine to fight against arthritis with the least hazardous side effects.
Aim: To evaluate the potential therapeutic antiarthritic effect of crocin, a dietary colorant carotenoid isolated from stigma of Crocus Sativus, in collagen induced arthritis in rats and to elucidate its possible underlying mechanisms.
Experimental Protocol: Rheumatoid arthritis had been established by intradermal injection of female albino rats with bovine collagen II emulsified in complete Freund’s adjuvant (day 0), then rats were given intradermal booster injection with bovine type II collagen 7 days after primary immunization. Rats were evaluated for arthritic severity by using a macro-scopic scoring system.
Material and Methods: From the day following the onset of arthritic symptoms, arthritic rats, with similar arthritic scores, were randomly categorized into three groups; rheuma-toid arthritis group, Ibuprofen treated rheumatoid arthritis group, crocin treated rheumatoid arthritis group. Treatment was continued for 14 days.
Evaluation of RA was performed by measuring erythrocyte sedimentation rate (ESR) and C-Reactive Protein (CRP) to asses disease activity and severity, serum level of Cartilage Oligomeric Matrix Protein (COMP), Matrix Metalloproteinase-3 (MMP-3), Acid Phosphatase were measured, inflammatory markers were estimated as Interleukin-6 (IL-6), Tumor Necrosis Factor-a (TNF-a) and Prostaglandin E2 (PGE-2), Superoxide Dismutase (SOD) and reduced Glutathione (GSH) as oxidative stress markers were also estimated.
Results: Arthritic rats showed increased arthritic score, increased ESR, CRP, cartilage and bone degradation that have been detected from increased serum levels of COMP and Acid phosphatase together with increased both inflammatory and oxidative stress markers when compared to normal control group. While, crocin administration, as well as Ibuprofen,